Gel Permeation Chromatography (GPC)

Gel Permeation Chromatography (GPC) also referred to as Size Exclusion Chromatography (SEC). THF ONLY BASED SYSTEM. Located in Olney G-12. Performed on a Waters Alliance 2695 HPLC system with PDA detector and refractive index detector. Uses Waters Styragel HR columns 1,3, and 4 for molecular weight determination of up to 600K. System uses 100% tetrahydrofuran (THF only) as the mobile phase at 0.9ml/min flow rate. Each sample must be dissolved in 100% tetrahydrofuran for 24 hours before analysis at 10 to 20 mg/ml, and filtered through a 0.45 micron nylon or PTFE filter. 105 sample carousel for automatic injections. Training takes 1-2 hours.