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microscopy

Fischione 2040 Dual-Axis Tomography Holder

By Fischione - 2040

Stanford University
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Features an optimal tilt angle range in narrow gap (~5 mm) pole-piece geometries, while maintaining microscope resolution. A fully jeweled mechanism provides ultra-precise, in-plane specimen rotation while maintaining eucentricity. Initially, the specimen can be fully rotated through 360º to orient either the grid bars or a specimen feature to the alpha tilt axis. Once the specimen is properly oriented, the first tilt series is acquired. A two-position precision indexing mechanism provides 90º in-plane rotation. These features greatly facilitate the acquisition of a dual-axis tilt series using SerialEM application for acquisition. Acquired images can then be opened using IMOD. IMOD can then create a three-dimensional tomogram.

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