FLIMer

Location Room 0084. Veritas Science Center (Former NRB) Imaging Modality Single point laser scanning confocal FLIM (fluorescence lifetime imaging - white light laser with pulse picker for capturing longer lifetimes) Multiplexing (>6 color imaging) Fixed and Live samples Transmitted light Live cell imaging (environmental enclosure with temperature control) Hardware Leica Inverted DMi8 Leica Stellaris 8 Falcon Motorized stage (ITK) Laser lines: 405 diode, WLL (440-790 tunable; pulse picker) Confocal detectors: 2 HydS, 2 HyDX, 1 HyDR, all spectral Full environmental enclosure for live imaging LasX acquisition software Objectives Plan Apo 10x/0.40 Air Fluotar 16x/0.6 multi-immersion. Correction collar from water to RI 1.57 Plan Apo 20x/0.75 Air Plan Apo 40x/1.25 Glycerol Plan Apo 63/1.40 Oil