BeckMan Coulter Analytical ultracentrifugation (AUC)

Applications • Examine sample purity • Molecular weight determination • Analysis of associating systems • Ligand binding and stoichiometries • Detection of conformational changes • Determine sedimentation and diffusion coefficients Sample requirements Analytical ultracentrifugation is a non-destructive technique. All samples can be recovered and used for further analysis. Sample and reference buffer need to be matched. When using interference optics, dialysis is required. For absorbance optics chromatography buffer is sufficient.