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Microscopy

Nikon Ti-2 Inverted Fluorescence Microscope

By Nikon - Ti-2

University of Nebraska-Lincoln
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It is equipped with automated stage and due digital cameras (Qi-@ mono with high sensitivity for low light/fluorescence, and F1-2 for colorimetric image). This system allows researchers to collect images using regular slides and dishes of cultured cells (or well plates) under sterile conditions for live cells imaging. The Ti-2 system has specific set of filter cubes (6 cube positions), including, DAPI, CFP, GFP, YFP, CY3/DsRed/RFP, mCherry/Texas Red, CY5, and CY7, allowing sequential image collections of samples with up to five fluorescent tags (including Cy7) with proper spectrum separation.

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