Widefield Microscopes

The Keck Center’s widefield microscope systems use fluorescent lamp excitation, filter cubes, and CCD monochrome and/or RGB-color cameras to produce fluorescent, brightfield, DIC, Phase Contrast, and/or RGB-color images. The motorized XY-stage on the Leica DMI6000 microscope allows for mosaic tiling of large tissue sections, both fluorescent and color-stained, and multi-point live imaging.  Widefield microscopes do not exclude out-of-focus light as scanning confocal microscopes do, so they are usually not suitable for imaging thick samples.