Zeiss LSM 700 Microscope

The Zeiss LSM 700 is equipped with four solid state lasers (405, 488, 555, and 639 nm). Available objectives include 10X dry, 20X dry, 40X oil, and 63X oil. Features of the Zeiss ZEN software include single image acquisition, z-stacks, tiling, programming of multiple locations, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Using the proprietary Zeiss software (ZEN) or freeware such as ImageJ or FIJI, one can assess intensity values within regions of interests (ROIs), create 3-D projections from z-stacks, generate histograms, and analyze images for co-localization.