Queen B

Location Room 1023. 4 Blackfan circle (HIM) Imaging Modality Phase Contrast Widefield Hardware Nikon Ti2-E inverted microscope Motorized filter turret Semrock Dichroics LED-CFP/YFP/mCherry-3X-A-000 LED-DA/FI/TR/Cy5/Cy7-5X-A-000 Motorized Emission Filter Wheel Semrock FF01-432/36 Semrock FF01-474/27 Semrock FF01-515/30 Semrock FF01-544/24 Semrock FF01-641/75 Semrock FF01-680/42 Lumencor Spectra III Light Engine illumination 390/22 440/20 475/28 510/25 555/28 575/25 637/12 – Laser 748/12 – Laser Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera Nikon Elements 5.2 Acquisition Software Objectives Plan Apo 100x/1.45 Oil Ph3 Plan Apo 60x/1.4 Oil Ph3 Plan Fluor 10x/0.3 Ph1 DLL Plan Apo λ 20x/0.75 DIC N2 Plan Apo VC 100x/1.4 Oil DIC N2 Plan Apo 4x/0.2