Leica Automated Freeze Substitution (AFS2) systems

The HCBI offers access to two EM Automated Freeze Substitution (AFS2) systems from Leica (Room B412.60, Northwest Building). The systems allow for manual or automated freeze substitution, progressive lowering of temperature (PLT), low temperature embedding and UV polymerization of resins. Both AFS systems are equipped with a freeze substitution processor (FSP) unit that automatically dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope allow for viewing and positioning of samples to ensure ease of use.