Nikon PRIMO

The Nikon PRIMO is a Nikon Ti-2 microscope with widefield fluorescence and an Alveole PRIMO attachment. The PRIMO is a micropatterning, hydrogel polymerization and microfabrication device that allows you to create custom made in vitro cellular microenvironments. It uses a digital micromirror device (DMD) technology that illuminates a pattern with UV light at 375nm.The Nikon Ti-2 is equipped with the D-LEDI fluorescent LED light source and Prime BSI Express Scientific CMOS (sCMOS) camera. The PRIMO also has a white LED light source and Nikon DS-Ri-2 color camera for bright field imaging of histology stained samples. The microscope offers a 22mm FOV image. Camera: Prime BSI Express Scientific CMOS (sCMOS) camera Nikon DS-Ri2 color camera Imaging Modes: PRIMO – micropatterning, hydrogel polymerization, and microfabrication Chromogenic microscopy (DAB, H&E, etc) Conventional fluorescence microscope Excitation Specs: Nikon D-LEDI LED light engine: 385 nm (DAPI) 475 nm (FITC. GFP) 550 nm (TRITC, Cy3) 621 nm (far red, Cy5) Objective Lenses: 4x Plan Fluor NA 0.13 WD 17.1mm 10x Plan Fluor DIC N1 NA 0.3 WD 16mm 20x Plan Apo λD OFN25 DIC N2 NA 0.8 WD 800um 20x S Plan Fluor ELWD DIC N1 NA 0.45 WD 8.2mm (for PRIMO) 40x Plan Fluor Oil DIC H N2 NA 1.3 WD 240um Charges: $35.00 / hour Note: To use the microscope you must have an active iLab account. Reservations are made through our on-line iLab calendar.Contact Tegy, or Oleg to arrange training. Location: MCN T-2216