Divine Stella

Location Room 0084. Veritas Science Center (Former NRB) Imaging Modality Single point laser scanning confocal Multiphoton imaging FLIM (confocal and multiphoton) Multiplexing (>6 color imaging) Lightning (enhanced resolution) Fixed and Live thick samples Transmitted light Long term live imaging (full environmental enclosure with CO2 or 95% O2 and active humidity) Objectives Plan Apo 10x/0.4 Air HC Fluotar L 16x/0.6 multi-immersion. Dipping. Correction collar for immersion from Water (RI 1.33) to RI 1.57 Can be used with BABB, iDISCO, Water based media, Glycerol Plan Apo 20x/0.75 multi-immersion Can be used With or Without Coverslip & with Water, Glycerol, Oil HC Apo L 40x/0.8 Water dipping only HC Apo L 63x/0.9 Water dipping only Plan Apo 63x/1.4 Oil only Plan Apo 25x/0.9 Water dipping only with motorized correction collar (single objective slider) Hardware Leica Upright DM6 Stellaris 8 Falcon DIVA Motorized stage (Scientifica) Laser lines (confocal): 405 nm diode, WLL (440-790 nm tunable; 80Hz pulsed) Laser Lines (multiphoton): Insight X3 dual beam (tunable 690-1300nm and fixed at 1040 nm) Variable Beam Expander to adjust multiphoton excitation volume (optimize to improve resolution or depth) Confocal detectors: 2 HyDS, 1 HyDX and 1 HyDR. All spectral 4Tune Spectra Non Descanned Spectral Detection (multiphoton): 1 multi-alkali PMT NDD, 2x HyD NDD Full environmental enclosure for live imaging (with active humidity) LasX acquisition software