Abberior STED Super-Resolution Microscope

3 color fluorescence imaging with resolution of 25-30nm Overview STED super-resolution microscopy (2014 Nobel Prize awarded to Stephan Hell) is a complex process which utilizes an excitation point, like a standard point-scan confocal, as well as a doughnut shaped pulse of intense light in the emission spectrum of the fluorophore. With the appropriately selected fluorophores, the doughnut shaped pulse briefly inactivates the fluorescence leaving only a sub-diffraction excited region at the center. The Abberior Facility STED system has many advantages over earlier STED systems. It uses pulsed excitation and depletion lasers which minimizes photo-toxicity and bleaching. Pulsed lasers also allow for fluorescence lifetime imaging (FLIM) either in confocal or STED mode. Sample preparation is critical for success. This involves the selection of appropriate fluorophores which can be briefly inactivated with the depletion doughnut as well as appropriate mounting media and coverglass selection. Please read more about Abberior microscopy on this website and contact us for assistance.