Leica SP8X STED/FLIM Microscope

The Leica 3x STED/FLIM system is a super resolution microscope based on the Leica SP8 confocal microscope. It has a guaranteed resolution of 100 nm though the system has been routinely achieving 50 nm. We recommend referencing the accepted dye list and sample prep notes by Leica for STED. The Fluorescent Life Time (FLIM) imaging has the ability to measure the decay rate of your probes. As the fluorescence lifetime does not depend on concentration, absorption by the sample, sample thickness, photo-bleaching and/or excitation intensity it is more robust than intensity based methods.